SPECIMEN REQUIREMENTS FOR FLOW CYTOMETRY

Flow Cytometry

Peripheral Blood

– 2 ml minimum

Sodium heparin (green top)

EDTA (purple top)

Include most recent CBC results

Bone Marrow Aspirate

– 2ml minimum

Sodium heparin (green top)

EDTA (purple top)

Include most recent CBC results

Cerebrospinal Fluid (CSF)

Fresh; Non-diluted; NO preservative

Tissue

RPMI

Body Fluids

Undiluted in sterile container

Cytogenetics

Peripheral Blood

– 2 ml minimum

– perform test within 2 – 3 days of draw

Sodium heparin (green top)

Include most recent CBC results

Bone Marrow Aspirate

– 2ml minimum

– perform test within 2 – 3 days of draw

Sodium heparin (green top)

Include most recent CBC results

Tissue

RPMI

FISH

Examples:

MDS

CLL

MYC

Peripheral Blood

– 2 ml minimum

Sodium heparin (green top)

Include most recent CBC results

Bone Marrow Aspirate

– 2ml minimum

Sodium heparin (green top)

Include most recent CBC results

Tissue

RPMI

PCR

Examples:

BCR/ABL

JAK2

FLT3

TCR

Peripheral Blood

– 2 ml minimum

– perform test within 2 – 3 days of draw

EDTA (purple top)

Include most recent CBC results

Bone Marrow Aspirate

– 2ml minimum

– perform test within 2 – 3 days of draw

EDTA (purple top)

Include most recent CBC results

Tissue

RPMI

PNH

Peripheral Blood – 2 ml minimum

EDTA (purple top)

Must be <24 hours old

Do NOT refrigerate

Include most recent CBC results