Pathology Associates of Tyler now offers in-house flow cytometry for the detection of leukemia and lymphoma. Our lab utilizes the state-of-the-art technology of an 8-color flow cytometer and a comprehensive panel of flourescent antibodies to test blood, bone marrow and tissue samples for the presence of hematopoietic and lymphoid malignancies.
Our flow cytometry laboratory is headed by Steven R. Sanchez, M.D., a fellowship-trained hematopathologist, assisted by flow cytometry technician Paula M. Wallace, M.S..
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SPECIMEN REQUIREMENTS FOR FLOW CYTOMETRY |
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Flow Cytometry |
Peripheral Blood – 2 ml minimum |
Sodium heparin (green top) EDTA (purple top) Include most recent CBC results |
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Bone Marrow Aspirate – 2ml minimum |
Sodium heparin (green top) EDTA (purple top) Include most recent CBC results |
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Cerebrospinal Fluid (CSF) |
Fresh; Non-diluted; NO preservative |
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Tissue |
RPMI |
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Body Fluids |
Undiluted in sterile container |
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Cytogenetics |
Peripheral Blood – 2 ml minimum – perform test within 2 – 3 days of draw |
Sodium heparin (green top) Include most recent CBC results |
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Bone Marrow Aspirate – 2ml minimum – perform test within 2 – 3 days of draw |
Sodium heparin (green top) Include most recent CBC results |
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Tissue |
RPMI |
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FISH
Examples: MDS CLL MYC |
Peripheral Blood – 2 ml minimum |
Sodium heparin (green top) Include most recent CBC results |
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Bone Marrow Aspirate – 2ml minimum |
Sodium heparin (green top) Include most recent CBC results |
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Tissue |
RPMI |
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PCR
Examples: BCR/ABL JAK2 FLT3 TCR |
Peripheral Blood – 2 ml minimum – perform test within 2 – 3 days of draw |
EDTA (purple top) Include most recent CBC results |
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Bone Marrow Aspirate – 2ml minimum – perform test within 2 – 3 days of draw |
EDTA (purple top) Include most recent CBC results |
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Tissue |
RPMI |
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PNH |
Peripheral Blood – 2 ml minimum |
EDTA (purple top) Must be <24 hours old Do NOT refrigerate Include most recent CBC results |
******Call Pathology Associates of Tyler (903-593-0481) for specimen pick as soon as specimen is collected*****